Composite

Part:BBa_K3722008:Design

Designed by: Gao Zhan   Group: iGEM21_NWU-CHINA-A   (2021-09-29)


T7_Promoter-RBS-MaFMO-T7_Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1435
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 633
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 213
    Illegal AgeI site found at 236
    Illegal AgeI site found at 870
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is more effective to use RBS than Linker.


Source

Synthesis

References